Agilent provides xCELLigence impedance-based, label-free, real time cell analysis system and NovoCyte flow cytometers.
Automated microscopy and Spatial Proteomics
Real-time, label free cell analysis
Nano and micro particle analysis
Accelerate to discover
Related topics
A 19-color single-tube Full Spectrum Flow Cytometry for the detection of Acute Myeloid Leukemia
Mar 13, 2024
This recent publication in Cytometry Part A describes the development and comprehensive workflow of a single-tube,...
18F-labeled somatostatin analogs for somatostatin receptors (SSTRs) targeted PET imaging of NETs
Mar 11, 2024
A novel 18F-radiolabeled somatostatin analogue, [Al18F]NODA-MPAA-HTA, was synthesized and evaluated for positron...
Discover Yokogawa CellVoyager CQ1: Benchtop High-Content Analysis System
Mar 8, 2024
Unlike flow cytometers, the CellVoyager CQ1 confocal quantitative image cytometer does not require pretreatment such as...
Real-time and quantitative analysis of Macrophage Phagocytosis with RTCA eSight
Feb 23, 2024
The eSight is currently the only instrument that interrogates cell health and behavior using cellular
impedance...
Visualization of Tumor-Related Blood Vessels in Small Animals by Photoacoustic 3D Imaging System
Feb 21, 2024
In Vivo Label-Free Observation of Tumor-Related Blood Vessels in Small Animals Using a Newly Designed Photoacoustic 3D...
Therapeutic effects of mesenchymal stem cells in myocardial infarction
Feb 19, 2024
Intrapericardial hydrogel injection generates high cell retention and augments therapeutic effects of mesenchymal stem...
Whole-slide biomarker quantitation in 82-patient NSCLC cohort study of acquired resistance to PD-(L)
Feb 7, 2024
Samples from 82 patients with NSCLC and matched pre- and post-ICI biopsies were subjected to genomic profiling and to...
Evaluation with BLI of synthetic immune checkpoint engagers in allogenic mice
Jan 17, 2024
Synthetic immune checkpoint engagers protect HLA-deficient iPSCs and derivatives from innate immune cell cytotoxicity
Nov 25, 2016
The search for novel anti-biofilm bio-active molecules is a very active research area for which simple, reliable, and fast screening methods are demanded. In this work we have successfully validated an impedance-based method, initially developed for the study of adherent eukaryotic cells, to monitor the formation of single-species biofilms of three model bacteria in real time. The xCelligence real time cell analyzer (RTCA) equipment uses specific microtiter E-plates coated with gold-microelectrodes that detect the attachment of adherent cells, thus modifying the impedance signal. In the current study, this technology allowed the distinction between biofilm-producers and non-producers of Staphylococcus aureus and Staphylococcus epidermidis, as well as the formation of Streptococcus mutans biofilms only when sucrose was present in the culture medium. Besides, different impedance values permitted discrimination among the biofilm-producing strains tested regardless of the nature of the polymeric biofilm matrix. Finally, we have continuously monitored the inhibition of staphylococcal biofilm formation by the bacteriophage phi-IPLA7 and the bacteriophage-encoded endolysin LysH5, as well as the removal of a preformed biofilm by this last antimicrobial treatment. Results observed with the impedance-based method showed high correlation with those obtained with standard approaches, such as crystal violet staining and bacteria enumeration, as well as with those obtained upon other abiotic surface (polystyrene and stainless steel). Therefore, this RTCA technology opens new opportunities in the biofilm research arena and its application could be further explored for other bacterial genera as well as for different bio-active molecules.
Related technologies: Real-time, label free cell analysis
Get more info
Brand profile
Agilent provides xCELLigence impedance-based, label-free, real time cell analysis system and NovoCyte flow cytometers.
More info at:
www.aceabio.com