Accelerate to discover

Back to filter

Related topics

Precision CellRad

Precision X-Ray

Feb 15, 2019

The CellRad system allows you to irradiate cells in your lab without compromising sterility or security. A smaller,...

New technique for dynamic monitoring of liquid tumor Cytotoxicity

Acea Biosciences

Feb 12, 2019

To develop and validate immuno-oncology therapies, scientists must understand the full dynamics of cytotoxic effectors...

Small animal 13C-hyperpolarized MRI

Bruker Biospin

Feb 8, 2019

13C-Hyperpolarized molecules provide a unique signature for molecular processes relevant to a range of disease...

Oncolytic chimeric orthopoxvirus causes regression of pancreatic cancer xenografts

Spectral Instruments Imaging

Jan 30, 2019

Pancreatic ductal adenocarcinoma has been increasing by 0.5% per year in the United States. This study describes the...

Feb 20, 2019

Etaluma can now offer integrated tablet for LS460/560/620 scopes

Etaluma

Jan 25, 2019

Based on the customer requests of tablet mount configuration for sterile picking in the hood, Etaluma can now offer the...

Responsive fluorescence probe for detection of Hypochlorous acid in live cells and animals

Spectral Instruments Imaging

Jan 17, 2019

In this work, a new fluorescence probe, PQI, was developed for monitoring of the HOCl level in biological samples. The...

Spectral data analysis webinar recording - Cytek flow cytometer and De Novo Software

Cytek Biosciences

Jan 17, 2019

Cytek Biosciences and De Novo Software are proud to announce a collaboration that allows you to work with your spectral...

Feb 20, 2019

Improved differentiation of Retinal Organoids stem cells derived using rotating-wall vessels

Synthecon

Jan 15, 2019

Pluripotent stem cells can be differentiated into 3D retinal organoids, with major cell types self-patterning into a...

Show all topics (10)

Neuronal co-culture - fluorescent live cell imaging

Aug 4, 2016

Neurite dynamics can be altered in disease states or following exposure to neurotoxic agents providing a rationale for understanding and quantifying this process (Conforti et al., 2007). An ideal method to track neurite dynamics would allow continuous automated measurement of structural parameters, including length and number of branch points, in a non-perturbing manner. We have previously developed a quantitative mono-culture approach for investigating changes in neurite length and ranching using phase images taken with an IncuCyte ZOOM live-content imaging platform in conjunction with NeuroTrack analysis software. However, in co-cultures containing neurons and astrocytes, neuronal processes cannot be unambiguously identified and measured using phase images alone. As such, we have developed a fluorescent reagent (NeuroLight Red) that specifically labels neurons in co-culture with astrocytes. Utilising a VSV-G pseudotyped lentivirus that encodes a red fluorescent protein (mKate2) driven off a synapsin promoter, neuronal expressionis strengthened and non-neuronal crossover is minimized. Here we describe assay methods using this fluorescent label, alongside NeuroTrack algorithms, to quantitatively assess changes in neurite morphology over time in either rat primary or human iPSC-derived neuron/astrocyte co-cultures.

Read more

 

Scientific paper
Application
Product news

Related technologies: Real-time, label free cell analysis

Get more info

Riccardo Pasculli

Head of application support

Riccardo

Pasculli

+420 731 127 717

Send Message

Brand profile

Sartorius

Essen BioScience (part of Sartorius), is a team of engineers and biologists with deep expertise in cell-based assays. We invent, manufacture, supply and support cell-based assay instrumentation, reagents and protocols.

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey