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Optical tweezers measurements of DNA conformational dynamics at the single-molecule level

Dec 4, 2017

DNA breathing, also known as fraying, describes the spontaneous local conformational transitions occurring within double-stranded DNA (dsDNA). Due to the transient and spontaneous nature of this process, the study of real-time DNA breathing transitions at the single-molecule level requires the use of highly sensitive experimentation technologies. Using LUMICKS’ high resolution optical tweezers, small and transient DNA conformational changes can be monitored in real-time, with an unprecedented stability, which provides scientists with valuable information on the number and frequency of various conformational stages.

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Regina Fillerová

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LUMICKS

Optical tweezers, Confocal microscopy, STED nanoscopy, Acoustic force spectroscopy for single-molecule biophysics. 

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Live, simultaneous and correlative visualization and manipulation of molecular interactions with sub-picoNewton force resolution and sub-nm position resolution.

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Acoustic Force Spectroscopy is a single-molecule, lab-on-a-chip technology in which acoustic waves are used to exert forces on thousands of biomolecules.

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