Accelerate to discover

Back to filter

Related topics

Anesthesia in small animals: How to optimize management?

RWD

Apr 22, 2021

Anesthesia is just a complex technique in which the administration of anesthetics drugs is the less important step.

Cytek webinar : Evaluating Spectral Cytometry for Immune Profiling in Viral Disease

Cytek Biosciences

Apr 19, 2021

In conventional fluorescence cytometry, each fluorophore in a panel is measured in a target detector, through the use...

Rethinking 3D cell culture with IncuCyte: advanced cell cultures from iPSCs and organoids

Sartorius

Apr 14, 2021

The world is neither flat nor two-dimensional, so why should cell culture be? Traditional cell culture systems grow...

May 12, 2021

Cytek webinar recording : Improved flow cytometry assay resolution with autofluorescence extraction

Cytek Biosciences

Mar 30, 2021

The power of autofluorescence extraction in the detection of dim fluorescence on highly autofluorescent cells, and the...

Bruker webinar : High-resolution exploration of ESM-based vascularized tissue-flaps

Bruker Biospin

Mar 22, 2021

By employing a tissue engineering approach based on biological tissue constructs, Dr. Redenski has fabricated composite...

May 12, 2021

ACCELA Educational webinar series : Cytek Aurora in action, how to easily resolve highly overlapping dyes ?

Cytek Biosciences

Mar 8, 2021

During this webinar, presented by Riccardo Pasculli, Head of Application Support @ ACCELA, we will cover: technology...

Show all topics (10)

Optimization of (CAR) T cell design guided by sensitive sssessment of B cell killing

Jul 10, 2017

Wednesday, July 12, 2017, 9 pm CEST.

Dan MacLeod Ph.D., Precision BioSciences, Inc.  

At Precision BioSciences, we have developed an allogeneic method using our proprietary ARCUS gene-editing technology to generate “universal” CAR T cells from healthy donors. These cells are engineered to eliminate expression of the endogenous T cell receptor making them safe for transfer to unrelated patients. By integrating the CAR transgene at a single genomic site, we can tightly control the level of CAR expression on the cell surface. We further optimize the cells by designing new CAR T constructs that include modifications to the extracellular binding domains as well as the intracellular signaling domains. Evaluating the impact of these new modifications requires sensitive methods to assay CAR T cell function. Traditional methods for evaluating cytotoxicity cannot effectively predict in vivo activity of a CAR T product; this is because they assess short-term killing potential rather than the ability to expand, persist, and exhibit serial killing. We have used the ACEA xCELLigence instrument for both B Cell Killing Assays and Leukemic Cell Killing Assays to test multiple CAR constructs with modified ectodomains, endodomains, and other modifications, and we have found this platform sensitive enough to detect subtle yet significant differences in performance not observed in traditional killing assays. Furthermore, the sensitivity of the instrument has enabled us to evaluate CAR constructs using cell numbers that are orders of magnitude lower than other assays, greatly enhancing our throughput and capabilities.

Register now

Webinar

Related technologies: Real-time, label free cell analysis

Get more info

Riccardo Pasculli

Head of application support

Riccardo

Pasculli

+420 731 127 717

Send Message

Brand profile

Agilent technologies

Agilent provides xCELLigence impedance-based, label-free, real time cell analysis system and NovoCyte flow cytometers.

Related products

Located inside a tissue culture incubator, the RTCA DP Analyzer has three integrated stations for E-Plates 16 or CIM-Plates 16

show detail

Located inside a tissue culture incubator, it is capable of switching any one of the wells on the E-Plate 96 to the RTCA Analyzer for impedance measurement

show detail

Located inside a tissue culture incubator, it is capable of switching any one of the wells on any of six E-Plates to the RTCA Analyzer for impedance measurement

show detail

Instrument can be integrated with automated liquid handling platforms or a robotic plate handler from different vendors

show detail

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey