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Webinar recording: Autofluorescence management - the power of Spectral Flow Cytometry

Cytek Biosciences

Jul 15, 2021

One of the key advantages of spectral flow cytometry is its ability to extract sample autofluorescence, making it much...

Webinar recording: Super-Resolution imaging of Chromatin organization in health and disease

ONI

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Understanding how cells organize the array of components within their membranous confines can not only provide insight...

Aug 1, 2021

PMOD Virtual User Meeting

Bruker Biospin

Jun 7, 2021

We look forward to welcoming many of our users to 2021 PMOD User Meeting. The theme of our PMOD User Meeting will be...

IncuCyte webinar recording: How to measure Immune Cell Killing of Tumor Cells effectively

Sartorius & Essen BioScience

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Immuno-oncology (IO) has transformed cancer treatment. The number of treatments in the IO pipeline continues to...

Aug 1, 2021

Bruker webinar: Experiences in The Use of Micro-CT in Insect Research

Bruker Biospin

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High resolution micro-CT is being used routinely in science. Mostly it is being used as a substitute to microscopy to...

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Optimization of (CAR) T cell design guided by sensitive sssessment of B cell killing

Jul 10, 2017

Wednesday, July 12, 2017, 9 pm CEST.

Dan MacLeod Ph.D., Precision BioSciences, Inc.  

At Precision BioSciences, we have developed an allogeneic method using our proprietary ARCUS gene-editing technology to generate “universal” CAR T cells from healthy donors. These cells are engineered to eliminate expression of the endogenous T cell receptor making them safe for transfer to unrelated patients. By integrating the CAR transgene at a single genomic site, we can tightly control the level of CAR expression on the cell surface. We further optimize the cells by designing new CAR T constructs that include modifications to the extracellular binding domains as well as the intracellular signaling domains. Evaluating the impact of these new modifications requires sensitive methods to assay CAR T cell function. Traditional methods for evaluating cytotoxicity cannot effectively predict in vivo activity of a CAR T product; this is because they assess short-term killing potential rather than the ability to expand, persist, and exhibit serial killing. We have used the ACEA xCELLigence instrument for both B Cell Killing Assays and Leukemic Cell Killing Assays to test multiple CAR constructs with modified ectodomains, endodomains, and other modifications, and we have found this platform sensitive enough to detect subtle yet significant differences in performance not observed in traditional killing assays. Furthermore, the sensitivity of the instrument has enabled us to evaluate CAR constructs using cell numbers that are orders of magnitude lower than other assays, greatly enhancing our throughput and capabilities.

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Webinar

Related technologies: Real-time, label free cell analysis

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Riccardo Pasculli

Head of application support

Riccardo

Pasculli

+420 731 127 717

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Agilent technologies

Agilent provides xCELLigence impedance-based, label-free, real time cell analysis system and NovoCyte flow cytometers.

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