Accelerate to discover

Back to filter

Related topics

IncuCyte webinar: high throughput approach to developing T- Cell immunotherapies

Sartorius

Jun 17, 2019

Immunotherapy for cancers involves artificially prompting the immune system to treat cancer. Many cancer cells have...

IncuCyte virtual event: Dimension of time - from culture to assay

Sartorius

Jun 5, 2019

This event offers an overview of how live-cell analysis enables real-time analysis for the full workflow of the cells....

Webinar recording: Detecting cancer-associated structural variants using Bionano optical maps

BioNano Genomics

May 15, 2019

Bionano’s genome mapping technology directly observes long molecules from 150,000 bp to megabase pair lengths to...

IncuCyte webinar recording: Human Neurons in vitro, next-gen models for neurological disease

Sartorius

Apr 23, 2019

The ability to generate and manipulate human neurons in vitro is an exciting advance that enables the validation,...

Jul 19, 2019

Rapid, Quantitative CEST Imaging with MRI Fingerprinting

Bruker Biospin

Apr 16, 2019

With applications spanning almost any disease area, from cancer, stroke, through to cardiac metabolism disorders, this...

Monitoring cancer treatments with immuno-PET webinar

Bruker Biospin

Mar 8, 2019

In this webinar, Dr Gabriela Kramer-Marek, Team Leader in Preclinical Molecular Imaging The Institute of Cancer...

Spectral data analysis webinar recording - Cytek flow cytometer and De Novo Software

Cytek Biosciences

Jan 17, 2019

Cytek Biosciences and De Novo Software are proud to announce a collaboration that allows you to work with your spectral...

Jul 19, 2019

IncuCyte webinar recording: CAR T-cell therapy, frontiers in cancer Immunotherapy

Sartorius

Jan 11, 2019

The success of immune checkpoint blockade adds new therapeutic category to Cancers are the most heterogenous group of...

Show all topics (10)

Optimization of (CAR) T cell design guided by sensitive sssessment of B cell killing

Jul 10, 2017

Wednesday, July 12, 2017, 9 pm CEST.

Dan MacLeod Ph.D., Precision BioSciences, Inc.  

At Precision BioSciences, we have developed an allogeneic method using our proprietary ARCUS gene-editing technology to generate “universal” CAR T cells from healthy donors. These cells are engineered to eliminate expression of the endogenous T cell receptor making them safe for transfer to unrelated patients. By integrating the CAR transgene at a single genomic site, we can tightly control the level of CAR expression on the cell surface. We further optimize the cells by designing new CAR T constructs that include modifications to the extracellular binding domains as well as the intracellular signaling domains. Evaluating the impact of these new modifications requires sensitive methods to assay CAR T cell function. Traditional methods for evaluating cytotoxicity cannot effectively predict in vivo activity of a CAR T product; this is because they assess short-term killing potential rather than the ability to expand, persist, and exhibit serial killing. We have used the ACEA xCELLigence instrument for both B Cell Killing Assays and Leukemic Cell Killing Assays to test multiple CAR constructs with modified ectodomains, endodomains, and other modifications, and we have found this platform sensitive enough to detect subtle yet significant differences in performance not observed in traditional killing assays. Furthermore, the sensitivity of the instrument has enabled us to evaluate CAR constructs using cell numbers that are orders of magnitude lower than other assays, greatly enhancing our throughput and capabilities.

Register now

Webinar

Related technologies: Real-time, label free cell analysis

Get more info

Riccardo Pasculli

Head of application support

Riccardo

Pasculli

+420 731 127 717

Send Message

Brand profile

Acea Biosciences

ACEA manufactures xCELLigence impedance-based, label-free, real time cell analysis system and NovoCyte flow cytometers.

Related products

The RTCA DP Analyzer has three integrated stations for E-Plates 16 or CIM-Plates 16

show detail

Located inside a tissue culture incubator, it is capable of switching any one of the wells on the E-Plate 96 to the RTCA Analyzer for impedance measurement

show detail

Located inside a tissue culture incubator, it is capable of switching any one of the wells on any of six E-Plates to the RTCA Analyzer for impedance measurement

show detail

Instrument can be integrated with automated liquid handling platforms or a robotic plate handler from different vendors

show detail

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey