Accelerate to discover

Back to filter

Related topics

Cytek webinar : Improved flow cytometry assay resolution with autofluorescence extraction

Cytek Biosciences

Mar 30, 2021

The power of autofluorescence extraction in the detection of dim fluorescence on highly autofluorescent cells, and the...

Unleash Your Illumina Sequencer with TELL-Seq Linked Reads

Sage Science

Mar 24, 2021

Sage Science distributes universal sequencing technology´s TELL-Seq linked-read NGS library prep kits used with very...

Bruker webinar : High-resolution exploration of ESM-based vascularized tissue-flaps

Bruker Biospin

Mar 22, 2021

By employing a tissue engineering approach based on biological tissue constructs, Dr. Redenski has fabricated composite...

Apr 12, 2021

A real time-saver, accurate cell counting in 15 seconds with C100 counter

RWD

Mar 17, 2021

Cell counters are tools for counting live and/or dead cells in a culture. Any researcher who works in a cell culture...

Innovation never takes a break with IncuCyte

Sartorius

Mar 11, 2021

Analyze your cells for days, weeks or even months as they sit stationary in the stable environment of your tissue...

Imaging Flow Cytometry Luminex learning sessions

Luminex

Mar 5, 2021

Start the new year with new knowledge! Get the most from your Amnis instrument and IDEAS Software with training...

Apr 12, 2021

Preclinical lung imaging offers effective evaluation of drug candidates

Bruker Biospin

Mar 1, 2021

The incidence of respiratory disease is increasing throughout the world, and the demand for safe, effective drugs to...

Show all topics (10)

Platelet GPIIb supports initial pulmonary retention but inhibits subsequent proliferation of melanom

Jul 28, 2017

In the present study, the research team dissected the role of platelet αIIb integrin (GPIIb) for early and late steps in pulmonary melanoma metastasis. They first addressed in vitro potential mechanisms for initial recruitment of circulating melanoma cells to vascular endothelium using a flow chamber model and then assessed the role of GPIIb for metastasis formation in vivo in mice lacking integrin αIIb (GPIIb-/-). GPIIb associates with GPIIIa (integrin β3) to form the platelet-specific integrin heterodimer GPIIb-IIIa (integrin αIIbβ3), representing the most abundant platelet surface receptor and predominantly functioning as platelet fibrinogen receptor. By binding to fibrinogen, but also to von Willebrand factor, GPIIb-IIIa mediates cross-linking of adjacent platelets, resulting in platelet aggregation and platelet secretion of chemokines as well as growth factors. Moreover, binding of GPIIb-IIIa to fibronectin, vitronectin or PECAM-1 leads to platelet adhesion to the vessel wall. In order to follow the initial steps of tumor metastasis in wildtype (WT) and GPIIb-deficient mice, they applied a novel microscopic approach using a fluorescence optical imaging system based on laser scanning confocal technology.

They showed that the acute retention of malignant melanoma cells is dramatically reduced in mice deficient in platelet GPIIb and also they found that GPIIb has a minor effect of adhesion of single melanoma cells, but rather mediates the formation of platelet-rich melanoma cell aggregates, which are retained in the pulmonary vasculature.

Read more

Scientific paper
Application

Related technologies: Probe based confocal microscopy

Get more info

Miroslav Vecheta

Support specialist

Miroslav

Vecheta

+420 255 700 886

Send Message

Brand profile

Mauna Kea Technologies

Mauna Kea Technologies manufactures Cellvizio Lab, confocal probe based imaging system, providing cellular-level images with minimal invasiveness for longitudinal studies.

Related products

Cellvizio, a fiber based microscope for observing in real time at cellular scale, features simultaneous, co-registered dual band excitation and detection

show detail

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey