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Target large genomic regions with 10x genomics + SageHLS

Oct 9, 2018

This approach, known as HLS-CATCH, allows users to apply long-range genomics to their gene or genomic region of interest, characterizing and phasing not only single nucleotide variants but also large structural variants including deletions, inversions, and translocations.
The HLS-CATCH+10x Genomics workflow is cost-effective. For instance, a 200Kb target can be isolated using HLS-CATCH with as few as two custom Cas9 complexes, whereas hybridization-capture methods would require hundreds to thousands of probes. In addition, because of the complementarity between the HLS-CATCH method and the 10x Chromium library system, sequencing reagent costs to achieve 100x phased target coverage are more than 10-fold lower than that required to obtain 100x phased whole genome data.

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Related technologies: Targeted DNA/protein size collection

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Regina Fillerová

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Sage Science

Preparative electrophoresis systems for targeted size selection of biomolecules.

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Targeted Size Selection for Next-Gen Sequencing (90bp – 1.5kb)

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Targeted Size Selection for Next-Gen Sequencing (90bp – 50kb)

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Automated size selection for 24 samples at a time.

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