Accelerate to discover

Back to filter

Related topics

Extracellular Vesicle Purity Enhanced by Gen 2 qEV Columns With Customised Proprietary Resin

IZON

May 4, 2022

Izon Science has announced the launch of Gen 2, the enhanced range of qEV columns for size-exclusion...

Multi-modal PET drives interdisciplinary preclinical imaging

Bruker Biospin

Feb 23, 2022

Preclinical imaging (PCI) plays a vital role in understanding the biological processes behind disease states at the...

Quantitative 3D Optical Imaging for Lago

Spectral Instruments Imaging

Feb 21, 2022

Spectral Instrument Imaging just announced a new, strategic partnership with InVivo Analytics, which enables...

Reduces fMRI-BOLD Response in the Brain

Bruker Biospin

Feb 18, 2022

fMRI studies often involve the electrical stimulation of i.e. the forepaw, detecting where this stimulation is...

May 28, 2022

Extensive assessment of Cytokine production on the NovoCyte Penteon flow cytometer

Agilent technologies

Nov 23, 2021

Cytokines are small molecules essential for immune cell response to activation by pathogens, autoimmunity, or...

X-ray-virus-inactivation

Precision X-Ray

Oct 20, 2021

Ionizing radiation approaches are effective but use of radioactive radioisotopic elements have become difficult to...

Duke uses XRAD 225 CX to test radiosensitivity hypothesis

Precision X-Ray

Oct 18, 2021

Image guided radiotherapy in a preclinical setting couldn’t be more useful than the treatment of brain tumors, where...

May 28, 2022

See What's Possible with the PippinHT

Sage Science

Sep 14, 2021

Programmable DNA size selection enables new discoveries by helping optimize the library detected by a sequencer. That’s...

Show all topics (10)

Timelapse imaging of Drosophila melanogaster larva's central nervous system

Feb 22, 2018

Researchers has been using various genetic manipulation tools such as microinjection to knock down certain genes or over-express proteins for visualizing the cellular activities. However, visualization of an intact-live neuronal response in larva's Central Nervous System (CNS) is challenging due to robust digging/burrowing behaviour that impedes neuroimaging. To address this problem, dissection is used to isolate and immobilize the CNS from the rest of the body. In order to obtain a true physiological response from the Drosophila CNS, it is important to avoid dissection, while the larva should be kept immobilized. In this paper, a series of microfluidic clamps were investigated for intact immobilization of the larva. As a result, an optimized structure for rapid mechanical immobilization of Drosophila larvae for CNS imaging was determined. The clamping and immobilization processes were characterized by imaging and movement measurement of the CNS through the expression of genetically encoded Calcium sensor GCaMP5 in all sensory and cholinergic interneurons. The optimal structure that included two 3D constrictions inside a narrowed channel considerably reduced the internal CNS capsule movements. It restricts the CNS movement to 10% of the motion from a glued larva and allows motion of only 10 ± 30 μm over 350 s immobilization which was sufficient for CNS imaging. These larva-on-a-chip platforms can be useful for studying CNS responses to sensory cues such as sound, light, chemosensory, tactile, and electric/magnetic fields.

Read more

 

Scientific paper

Related technologies: Live cell imaging

Get more info

Riccardo Pasculli

Head of application support

Riccardo

Pasculli

+420 731 127 717

Send Message

Brand profile

Etaluma

With a focus on portability, ease of use, high-quality imaging and fast frame-rate, Etaluma developed new concept in simple, affordable, and accessible fluorescence microscopy.

Related products

The true color inverted brightfield microscopy with all of the LumaView software features of the fluorescent LumaScope

show detail

The original single color 488nm excitation fluorescence inverted microscopy

show detail

Lumascope 620 offers a larger sensor and 3-color fluorescence for the most powerful microscope offered anywhere at this price

show detail

Blue, green & red fluorescence and walk-away automation

show detail

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey