Accelerate to discover

Back to filter

Related topics

Imaging Performance of a multimodal module to enhance preclinical irradiator capabilities

Precision X-Ray

Sep 15, 2020

Upgrading an existing irradiator with such a multi-modal imaging device is a reasonable investment in laboratories...

The Role of Micro-CT in Imaging Breast Cancer Specimens

Bruker Biospin

Jun 17, 2020

The goal of breast cancer surgery is to remove all of the cancer with a minimum of normal tissue, but absence of full...

Bruker´s micro-CT used for some of the World’s First in vivo COVID-19 Imaging Experiments

Bruker Biospin

Jun 9, 2020

Bruker's BioSpin PreClinical Imaging Division reports the use of the Bruker SkyScan 1278 in vivo micro-CT system at the...

A mouse model of undifferentiated pleomorphic sarcoma mediated by adeno-Cre injection

Spectral Instruments Imaging

Jun 3, 2020

Soft tissue sarcomas pose a significant clinical challenge, with approximately 12,000 new diagnoses and an estimated...

Sep 19, 2020

Immunophenotyping Extracellular Vesicles using Amnis CellStream

Luminex

May 29, 2020

Attempts to analyze EVs using traditional PMT- based flow cytometers has been hampered by the limit of detection of...

Four-week individual caging of male ICR mice alters body composition without change in body mass

MEDIKORS

May 25, 2020

Could individual caging affect the physical composition of outbred mice? To investigate this, dual X-ray absorptiometry...

Detect structural variants at 1% allele fraction: Dive deeper into heterogeneous cancer samples

BioNano Genomics

May 22, 2020

To get a complete picture of highly rearranged cancer genomes in heterogeneous samples, use Bionano genome imaging...

Sep 19, 2020

Insulin receptor substrate 1 is a substrate of the Pim protein kinases

Spectral Instruments Imaging

May 18, 2020

The goal of these studies was to identify Pim substrate(s) that could help define the pathway regulated by these...

Show all topics (10)

Timelapse imaging of Drosophila melanogaster larva's central nervous system

Feb 22, 2018

Researchers has been using various genetic manipulation tools such as microinjection to knock down certain genes or over-express proteins for visualizing the cellular activities. However, visualization of an intact-live neuronal response in larva's Central Nervous System (CNS) is challenging due to robust digging/burrowing behaviour that impedes neuroimaging. To address this problem, dissection is used to isolate and immobilize the CNS from the rest of the body. In order to obtain a true physiological response from the Drosophila CNS, it is important to avoid dissection, while the larva should be kept immobilized. In this paper, a series of microfluidic clamps were investigated for intact immobilization of the larva. As a result, an optimized structure for rapid mechanical immobilization of Drosophila larvae for CNS imaging was determined. The clamping and immobilization processes were characterized by imaging and movement measurement of the CNS through the expression of genetically encoded Calcium sensor GCaMP5 in all sensory and cholinergic interneurons. The optimal structure that included two 3D constrictions inside a narrowed channel considerably reduced the internal CNS capsule movements. It restricts the CNS movement to 10% of the motion from a glued larva and allows motion of only 10 ± 30 μm over 350 s immobilization which was sufficient for CNS imaging. These larva-on-a-chip platforms can be useful for studying CNS responses to sensory cues such as sound, light, chemosensory, tactile, and electric/magnetic fields.

Read more

 

Scientific paper

Related technologies: Live cell imaging

Get more info

Riccardo Pasculli

Head of application support

Riccardo

Pasculli

+420 731 127 717

Send Message

Brand profile

Etaluma

With a focus on portability, ease of use, high-quality imaging and fast frame-rate, Etaluma developed new concept in simple, affordable, and accessible fluorescence microscopy.

Related products

The true color inverted brightfield microscopy with all of the LumaView software features of the fluorescent LumaScope

show detail

The original single color 488nm excitation fluorescence inverted microscopy

show detail

Lumascope 620 offers a larger sensor and 3-color fluorescence for the most powerful microscope offered anywhere at this price

show detail

Blue, green & red fluorescence and walk-away automation

show detail

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey