Accelerate to discover

Back to filter

Related topics

Development of superparamagnetic nanoparticles as an efficient contrast agent for multimodal imaging

Bruker Biospin

Jan 16, 2020

Gadolinium‐based contrast agents are used to provide diagnostic information in clinical magnetic resonance...

IncuCyte live-cell analysis system for live-cell visualization of neuronal activity

Sartorius

Jan 13, 2020

A major impediment to studying diseases affecting the human nervous system is the ability to monitor, analyze, and...

Latest updates to Bionano Access and Bionano Solve

BioNano Genomics

Jan 8, 2020

Bionano Access v1.5 and Bionano Solve v3.5 are the latest updates to Bionano visualization software and analysis tools....

The importance of titrating Viability dyes on the Aurora Spectral Cytometer

Cytek Biosciences

Jan 2, 2020

What is the best way to decide when your viability is titrated properly? Unlike titrating antibodies how to determine...

Jan 18, 2020

New analysis open modules expand capabilities of Guava Muse flow cytometer

Luminex

Dec 30, 2019

Sophisticated cell analysis doesn’t have to be complicated or costly. With the Guava Muse cell analyzer, you can now...

High molecular weight DNA from bone marrow aspirates samples in 4 hrs

BioNano Genomics

Dec 23, 2019

With Bionano Prep SP BMA DNA Isolation kit, transform the way you are isolating UHMW DNA from this complex sample type...

Excellent resolution of small particles using NovoCyte Quanteon flow cytometer

Acea Biosciences

Dec 18, 2019

The importance of cell-derived EVs has been recognized in multiple biological processes, and is a topic of increasing...

Jan 18, 2020

Cutting-edge analysis of Extracellular Microparticles using ImageStream Imaging Flow Cytometry

Amnis

Dec 16, 2019

Interest in extracellular vesicle biology has exploded in the past decade, since these microstructures seem endowed...

Show all topics (10)

xCELLigence in personalized cancer medicine

Dec 8, 2016

The use of patients' own cancer cells for in vitro selection of the most promising treatment is an attractive concept in personalized medicine. Human carcinoma cells from malignant pleural effusions (MPEs) are suited for this purpose since they have already adapted to the liquid environment in the patient and do not depend on a stromal cell compartment. Aim of this study was to develop a systematic approach for the in-vitro culture of MPEs to analyze the effect of chemotherapeutic as well as targeted drugs.

Methods: MPEs from patients with solid tumors were selected for this study. After morphological and molecular characterization, they were cultured in medium supplemented with patient-derived sterile-filtered effusion supernatant. Growth characteristics were monitored in real-time using the xCELLigence system. MPEs were treated with a targeted therapeutic (erlotinib) according to the mutational status or chemotherapeutics based on the recommendation of the oncologists. Results: We have established a robust system for the ex-vivo culture of MPEs and the application of drug tests in-vitro. The use of an antibody based magnetic cell separation system for epithelial cells before culture allowed treatment of effusions with only moderate tumor cell proportion. Experiments using drugs and drug-combinations revealed dose-dependent and specific growth inhibitory effects of targeted drugs.

Conclusions: We developed a new approach for the ex-vivo culture of MPEs and the application of drug tests in-vitro using real-time measuring of cell growth, which precisely reproduced the effect of clinically established treatments by standard chemotherapy and targeted drugs. This sets the stage for future studies testing agents against specific targets from genomic profiling of metastatic tumor cells and multiple drug-combinations in a personalized manner.

Read more

Scientific paper
Application

Related technologies: Real-time, label free cell analysis

Get more info

Riccardo Pasculli

Head of application support

Riccardo

Pasculli

+420 731 127 717

Send Message

Brand profile

Acea Biosciences

ACEA manufactures xCELLigence impedance-based, label-free, real time cell analysis system and NovoCyte flow cytometers.

Related products

Located inside a tissue culture incubator, the RTCA DP Analyzer has three integrated stations for E-Plates 16 or CIM-Plates 16

show detail

Located inside a tissue culture incubator, it is capable of switching any one of the wells on the E-Plate 96 to the RTCA Analyzer for impedance measurement

show detail

Located inside a tissue culture incubator, it is capable of switching any one of the wells on any of six E-Plates to the RTCA Analyzer for impedance measurement

show detail

Instrument can be integrated with automated liquid handling platforms or a robotic plate handler from different vendors

show detail

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey