Accelerate to Discover

Back to filter

Related topics

How to build the largest genome ever, in 3 easy steps

BioNano Genomics

Feb 23, 2018

Here we report the sequencing and assembly of the 32-gigabase-pair axolotl genome using an approach that combined...

Timelapse imaging of Drosophila melanogaster larva's central nervous system

Etaluma

Feb 22, 2018

Drosophila melanogaster is a well-established model organism to understand biological processes and study human...

High-throughput observation of molecular motor activity & dynamics

LUMICKS

Feb 21, 2018

With LUMICKS’ C-Trap, it is possible to observe myosin activity,the processive motion, the binding kinetics along...

A novel real-time CTL assay to measure designer T-cell function against HIV Env(+) cells

Acea Biosciences

Feb 19, 2018

To increase the immunosurveillance in HIV infection, we used retroviral vectors expressing CD4-chimeric antigen...

Feb 25, 2018

xxcloud – thermal cycler software

BJS Biotechnologies

Feb 16, 2018

Tired of waiting in line to use a qPCR system? The wait is over… the xxcloud is here! The xxcloud is exciting new...

BioNano mapping outperforms existing clinical tests in Duchenne´s muscular dystrophy patients

BioNano Genomics

Feb 15, 2018

In a publication in Genome Medicine, a team of researchers from Children’s National Health Center and University of...

Label-free, real-time live-cell assays for spheroids: IncuCyte bright-field analysis

Sartorius

Feb 12, 2018

A  growing  body  of  evidence  suggests  that  more  relevant  and  translational  observations  can  be  made  with ...

Feb 25, 2018

Measuring absolute blood perfusion in mice using dynamic contrast-enhanced ultrasound

VisualSonics FujiFilm

Feb 8, 2018

Contrast-enhanced ultrasound imaging involves the injection of gas-filled microbubbles that do not extravasate. This...

Show all topics (10)

Scaling to singles - summary of single cell mRNAseq methods

Jan 20, 2017

Fluidigm’s C1 system is encased in a benchtop machine that automatically orchestrates the steps of Smart-seq, taking your cell suspension and isolating and lysing cells, reverse transcribing their mRNA, and amplifying the resulting cDNA. It requires nonreusable microfluidic array chips that analyze 96 cells. Amplified cDNA is then sequenced or detected using qPCR. Switching mechanism at 5′ end of the RNA transcript (Smart-seq) is one of the only sequencing protocols that allows you to generate full-length coverage of transcriptomes from single cells, which is important if you’re studying allele-specific gene expression or splice variants.

Read more

Scientific paper
Technical breakthrough
Application

Related technologies: Automated Single-cell Genomics

Get more info

Regina Fillerová

Field Application Specialist

Regina

Fillerová

+420 731 127 718

Send Message

Brand profile

Fluidigm

Fluidigm is a Molecular and Single Cell Biology company that creates microfluidic-based chips and instrumentation for Life-Science research.

Related products

The first automated solution for single-cell genomics, now capable of even more

show detail

The system for precise manipulation of biological pathways for in vitro cell culture.

show detail

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey