Using in vivo two-photon time-lapse imaging of PGUSG larvae, we were able to monitor the synapse behaviour on developing RGC axonal arbors over hours, days or even weeks. We then developed methods to simultaneously image both pre- and post-synaptic compartments at putative synaptic connections in vivo. Finally, using reverse genetic manipulation on PGUSG larvae, we showed
that miR-132 plays a role in developmental synaptogenesis.
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Article was published in Nature, on 19 September 2018. Authors: Xu-fei Du, Bing Xu, Yu Zhang, Min-jia Chen & Jiu-lin Du. (2018) 8:14077 | DOI:10.1038/s41598-018-32409-y