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Unleash Your Illumina Sequencer with TELL-Seq Linked Reads

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Sage Science distributes universal sequencing technology´s TELL-Seq linked-read NGS library prep kits used with very...

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Cesium-137 versus X-ray irradiation preconditioning in immunodeficient NOG mice

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Radio-active sources have been used routinely for the preconditioning in humanized mouse models, but safety issues have...

Don’t Let Waste Anesthetic Gases Harm You!

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Many projects have been devoted to the study of the correlation between anesthetics and Alzheimer’s disease....

Advances in Leukemia research using shear flow and Bioflux system

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Leukemia is a rare cancer with many subtypes. The production of abnormal leukocytes create disruptions in the immune...

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Do you have our CellRad benchtop irradiator? It’s the only one on the market!

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The CellRad is a smaller, simpler, safer, and more cost effective alternative to radioisotope or high-powered X-ray...

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De novo sequence assembly using nanochannel arrays from BioNano Genomics

Apr 4, 2016

Next-generation sequencing (NGS) technologies have enabled high-throughput and low-cost generation of sequence data;however, de novo genome assembly remains a great challenge, particularly for large genomes. NGS short reads are often insufficient to create large contigs that span repeat sequences and to facilitate unambiguous assembly. Plant genomes are notorious for containing high quantities of repetitive elements, which combined with huge genome sizes, makes accurate assembly of these large and complex genomes intractable thus far. Using two-color genome mapping of tiling bacterial artificial chromosomes (BAC) clones on nanochannel arrays delivered by BioNano Genomics, scientists completed high-confidence assembly of a 2.1-Mb, highly repetitive region in the large and complex genome of Aegilops tauschii.

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BioNano Genomics

A revolutionary NanoChannel technology for Genome mapping of extremely long DNA without amplification, providing long-range contiguity and eliminating PCR bias.

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Genome maps of extremely long DNA without amplification, providing long-range contiguity and eliminating PCR bias

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