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How to build the largest genome ever, in 3 easy steps

Feb 23, 2018

The axolotl is an odd creature. It never really grows up and stays in its larval stage throughout life. It looks like an anime character. It regenerates limbs effortlessly. And it has the largest genome ever assembled: 32 gigabasepairs – that’s ten times the size of a human genome.
Where do you even start to assemble such a monstrously large genome? You take 3 easy steps:
Step 1, you use Bionano’s high throughput Saphyr to build 2 complete maps of the genome, based on separate labeling of two sequence motifs.
Step 2, you use these maps to anchor sequence contigs generated from 32x PacBio coverage using a custom built assembler, error corrected with 7x Illumina short reads.
Step 3, you use the Bionano maps to resolve 4,828 chimeric contigs. And to be honest, that happens automatically as part of step 2.

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BioNano Genomics

A revolutionary NanoChannel technology for Genome mapping of extremely long DNA without amplification, providing long-range contiguity and eliminating PCR bias.

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Genome maps of extremely long DNA without amplification, providing long-range contiguity and eliminating PCR bias

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Saphyr provides rapid, high-throughput, long-range genome mapping with unmatched structural variation discovery capabilities

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