Experimental studies on germ free animals in most institutions are typically performed in sterile isolators. These studies are often short term (2 – 12 weeks) and usually only utilize a small number of cages (2 – 6). For the facilities this means breaking down and recycling several isolators a month resulting in increased labor for the facility staff and ‘down time’ for investigators. The process of breaking down, recycling and testing is time consuming and can take up to 3 weeks. Not only does this process affect investigator’s ability to start their next experiment, it requires a good deal of a technician’s time, which could be spent in other ways. With so many negatives associated with this process, the authors decided to see if they could maintain germ free animals on a conventional mouse rack. The answer is yes!
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