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Low shear micro-gravity, a better way to do 3D cell culture

Aug 20, 2018

The in vitro culture of cells has played a central role in the biological sciences dating back to the beginning of the 20thcentury. Yet, for all the advanced tools that have been developed for studying cells, the method for culturing cells has remained virtually unchanged. The classical method for culturing cells involves growing cells as two-dimensional monolayers on flat, impermeable supports made of plastic or glass. While this method contributed much to our understanding of basic cell biology, it falls short of recapitulating in vivo structure and function of differentiated tissues. The key component that is missing in monolayer culture is growth in 3D. It has long been appreciated that the culture of cells in 2D monolayers leads to a rapid loss of the differentiated phenotype, but only in the last 10-15 years have methods to culture cells in 3D come into more widespread use.

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Riccardo Pasculli

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Synthecon

Inovating 3D cell culture technology exploring the rotary culture system for tissue engineering, stem cell, tumor spheroid and more. Unique Rotary Cell Culture System (RCCS)  for 3D culturing both suspension and anchorage-dependent cells.

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The disposable (single use) vessel culture systems are low cost, easy to use, rotary bioreactor systems that utilize disposable culture vessels to enable 3D cell culture.

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The autoclavable RCCS use culture vessels designed for repeated autoclaving, and ideal for long-term use. Suitable for any of the Synthecon culture vessels supplied with variable speed motor drives equipped with tachometer.

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The RCCS-SC systems are a unique 3D cell culture technology with scaled down size for economic and more efficient stem cells growth.

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RCCMAX allows undisturbed 3D cultivation of cells in vitro condition with continuous supplementation of fresh media and oxygen.

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