Accelerate to discover

Back to filter

Related topics

Build your experiments online with new Spectrum Viewer and SpectroFlo Experiment Builder

Cytek Biosciences

May 5, 2021

Explore new capabilities of Cytek's Full Spectrum Viewer and complementary SpectroFlo Experiment Builder. Explore on...

IVM-MS2: the most compact IntraVital Two-Photon microscope in the world

IVIM Technology

Apr 28, 2021

IVIM Technology, Inc. has released the most compact all-in-one intravital two-photon microscope. IVM-MS2 is the world's...

Cytek Spectral cytometers : taking science one step further with 200+ Publications

Cytek Biosciences

Apr 26, 2021

Cytek Aurora is helping researchers around the world take their science further. See how in the 200+ publications in...

Anesthesia in small animals: How to optimize management?

RWD

Apr 22, 2021

Anesthesia is just a complex technique in which the administration of anesthetics drugs is the less important step.

May 8, 2021

Cytek webinar : Evaluating Spectral Cytometry for Immune Profiling in Viral Disease

Cytek Biosciences

Apr 19, 2021

In conventional fluorescence cytometry, each fluorophore in a panel is measured in a target detector, through the use...

Rethinking 3D cell culture with IncuCyte: advanced cell cultures from iPSCs and organoids

Sartorius

Apr 14, 2021

The world is neither flat nor two-dimensional, so why should cell culture be? Traditional cell culture systems grow...

May 8, 2021

The impact of environmental Oxygen content on Natural Killer Cell Immunotherapy

Xcell Biosciences

Apr 13, 2021

Natural Killer (NK) cells are being studied in the clinic in a number of settings, including solid tumor...

Show all topics (10)

Next generation mapping reveals novel large genomic rearrangements in prostate cancer

Mar 30, 2017

Complex genomic rearrangements are common molecular events driving prostate carcinogenesis. In this study, we sought to determine the feasibility of using the Irys (Bionano Genomics Inc.) nanochannel NGM technology to generate whole genome maps of a primary prostate tumor and matched blood from a Gleason score 7 (4 + 3), ETS-fusion negative prostate cancer patient. With an effective mapped coverage of 35X and sequence coverage of 60X, and an estimated 43% tumor purity, we identified 85 large somatic structural rearrangements and 6,172 smaller somatic variants, respectively. The vast majority of the large SVs (89%), of which 73% are insertions, were not detectable ab initio using high-coverage short-read NGS. However, guided manual inspection of single NGS reads and de novo assembled scaffolds of NGM-derived candidate regions allowed for confirmation of 94% of these large SVs, with over a third impacting genes with oncogenic potential. From this single-patient study, the first cancer study to integrate NGS and NGM data, we hypothesise that there exists a novel spectrum of large genomic rearrangements in prostate cancer, that these large genomic rearrangements are likely early events in tumorigenesis, and they have potential to enhance taxonomy.

Read more

 

Scientific paper
Application

Get more info

Regina Fillerová

Field application specialist

Regina

Fillerová

+420 731 127 718

Send Message

Brand profile

BioNano Genomics

A revolutionary NanoChannel technology for Genome mapping of extremely long DNA without amplification, providing long-range contiguity and eliminating PCR bias.

Related products

Genome maps of extremely long DNA without amplification, providing long-range contiguity and eliminating PCR bias

show detail

Saphyr provides rapid, high-throughput, long-range genome mapping with unmatched structural variation discovery capabilities

show detail

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey