Accelerate to discover

Back to filter

Related topics

Travel grant ALERT! Submit ESHG abstracts today

BioNano Genomics

Feb 13, 2020

Interested in sharing your Bionano Genomics data at ESHG 2020 in Berlin? Eager to share how you have utilized the...

AutoLV analysis software

VisualSonics FujiFilm

Feb 11, 2020

AutoLV analysis software is the latest addition to VisualSonics robust suite of measurement tools customized for...

CellPathfinder Software, High Content Analysis and powerful Deep Learning features

Yokogawa

Feb 7, 2020

The intuitive CellPathfinder Software guides the user throughout the process. It analyzes and digitizes complex...

COY - personalized hypoxic and anaerobic chambers since 1969

COY laboratory products

Jan 31, 2020

When your research requires consistent atmospheric control to maintain anoxia, hypoxia, normoxia, or hyperoxia, you can...

Feb 18, 2020

Metabolic MR Imaging of Obesity

Bruker Biospin

Jan 29, 2020

Obesity is now known to be much more than a cosmetic problem with its association with a wide plethora of diseases...

Micro-CT in 3D data integration and (Clinical) workflows for digital orthodontics

Bruker Biospin

Jan 29, 2020

Petra Julia Koch and Paul Zaslansky (Charité - Universitätsmedizin Berlin) will discuss the importance of 3D data in...

Bionano webinar recording : EnFocus FSHD analysis

BioNano Genomics

Jan 27, 2020

Bionano introduced novel and reliable molecular method for facioscapulohumeral muscular dystrophy detection and fast...

Feb 18, 2020

Micro-CT study of the anatomy of a grasshopper

Bruker Biospin

Jan 23, 2020

Scanned with a Bruker Skyscan-1172 high resolution microtomograph at the Department of Zoology of the University of...

Show all topics (10)

Whole genome optical mapping reveals multiple fusion events chained by novel sequences in cancer

Sep 5, 2017

Genomic rearrangements are common in cancer, with demonstrated links to disease progression and treatment response. These rearrangements can be complex, resulting in fusions of multiple chromosomal fragments and generation of derivative chromosomes. While methods exist for detecting individual fusions, they are generally unable to reconstruct complex chained events. To overcome these limitations, we adopted a new optical mapping approach, allowing for megabase length DNA to be captured, and in turn rearranged genomes to be visualized without loss of integrity. Whole genome mapping (Bionano Genomics) of a well-studied highly rearranged liposarcoma cell line, resulted in 3,338 assembled haploid genome maps, including 101 fusion maps. These fusion maps represent 175 Mb of highly rearranged genomic regions, illuminating the complex architecture of chained fusions, including content, order, orientation, and size. Spanning the junction of 151 chromosomal translocations, we found a total of 32 Mb of novel interspersed sequences that were not detected from short-read sequencing. We demonstrate that optical mapping provides a powerful new approach for capturing a higher level of complex genomic architecture, creating a scaffold for renewed interpretation of sequencing data of particular relevance to human cancer.

Read more

 

Scientific paper
Application

Related technologies: Genome mapping

Get more info

Regina Fillerová

Field application specialist

Regina

Fillerová

+420 731 127 718

Send Message

Brand profile

BioNano Genomics

A revolutionary NanoChannel technology for Genome mapping of extremely long DNA without amplification, providing long-range contiguity and eliminating PCR bias.

Related products

Genome maps of extremely long DNA without amplification, providing long-range contiguity and eliminating PCR bias

show detail

Saphyr provides rapid, high-throughput, long-range genome mapping with unmatched structural variation discovery capabilities

show detail

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey