Accelerate to discover

Back to filter

Related topics

Easy and accurate cell counting in less than 20 seconds with C100 counter

RWD

Oct 16, 2020

Cell counters are tools for counting live and/or dead cells in a culture. Any researcher who works in a cell culture...

Automated fever & hypothermia detection

emka TECHNOLOGIES

Oct 14, 2020

As one of the oldest clinical indicators of disease in mammals, temperature is a crucial parameter to monitor,...

Multi-modal molecular imaging driving pre-clinical research & pharmaceutical development

Bruker Biospin

Oct 6, 2020

Multi-modal molecular imaging at Massachusetts General Hospital – driving pre-clinical research and patient pathways...

Developing tools for multiplex to single EV analysis pipelines

Cytek Biosciences

Sep 30, 2020

Extracellular vesicles (EVs) are lipid spheres released from cells. EVs express proteins and contain cargo that pertain...

Oct 20, 2020

IncuCyte webinar : clonal heterogeneity promotes metabolite exchange to support pancreatic tumor

Sartorius

Sep 25, 2020

Pancreatic ductaladenocarcinoma (PDA) is the third leading cause of cancer related death in the United States,...

Why ventilator is so important in the battle against COVID-19?

RWD

Sep 21, 2020

The COVID-19 has caused shortages of medical devices in many countries, especially ventilators. Many companies around...

Imaging Performance of a multimodal module to enhance preclinical irradiator capabilities

Precision X-Ray

Sep 15, 2020

Upgrading an existing irradiator with such a multi-modal imaging device is a reasonable investment in laboratories...

Oct 20, 2020

Are you still using traditional methodologies for your Oncology cell metabolism studies?

Sartorius

Sep 10, 2020

In this infographic, learn about the challenges experienced by scientists in analysing cellular metabolism and how...

Show all topics (10)

A mouse model of undifferentiated pleomorphic sarcoma mediated by adeno-Cre injection

Jun 3, 2020

Genetic mouse models of soft tissue sarcoma provide critical insights into disease pathophysiology, which are oftentimes unable to be extracted from human tumor samples or xenograft models. In this study we describe a mouse model of soft tissue sarcoma mediated by adenoviral-Cre recombinase injection into Trp53fl/fl/Ptenfl/fl lox-stop-lox luciferase mice. Injection of adenovirus expressing Cre recombinase, either subcutaneously or intramuscularly in two experimental groups, results in viral infection and gene recombination with 100% penetrance within the first 24 hours following injection. Luciferase expression measured by real-time bioluminescence imaging increases over time, with an initial robust increase following viral injection, followed by a steady rise over the next several weeks as primary tumors develop and grow. Intramuscular injections were more commonly associated with evidence of systemic viral distribution than subcutaneous injections. All mice developed soft tissue sarcomas at the primary injection site, with histological examination identifying 93% of tumors as invasive pleomorphic sarcomas based on microscopic morphology and immunohistochemical expression of sarcoma markers. A lymphocytic infiltrate was present in 64% of the sarcomas in this immunocompetent model and 71% of tumors expressed PD-L1. The bioluminescence imaging feature, along with high penetrance of the model and its immunological characteristics, makes it suited for pre-clinical studies of soft tissue sarcoma.

Do you want to learn more? Read the whole article!

Scientific paper
Application

Get more info

Miroslav Vecheta

Support specialist

Miroslav

Vecheta

+420 255 700 886

Send Message

Brand profile

Spectral Instruments Imaging

Spectral Instruments Imaging manufactures instruments for preclinical optical (bioluminescent, fluorescent) and X-ray imaging.

Related products

System for individual researchers and smaller specialist teams to conduct optical imaging studies in disease model progression, response to therapy, and cell migration in-vivo.

show detail

The Ami HT and Ami HTX (includes X-Ray imaging) optical imaging systems establish a new, high throughput benchmark for in vivo imaging suitable for specialist researchers and small teams.

show detail

The Lago and X‐Ray capable Lago X optical imaging systems provide a powerful and flexible in-vivo imaging capability suitable for imaging cores, specialist researchers and small teams, delivering an unmatched 10 mouse capacity across BLI, FLI and X‐Ray.

show detail

We supply and support Life-Science Technologies in the territory of Central and Eastern Europe.

Czech Republic

Slovakia

Hungary

Poland

Croatia

Slovenia

Serbia

Romania

Bulgaria

Latvia

Lithuania

Estonia

Russia

Ukraine

Belarus

Turkey